Expression of tissue factor procoagulant activity: regulation by cytosolic calcium.

Expression of tissue factor procoagulant activity: regulation by cytosolic calcium.

Intact bovine fibroblasts, pericytes, and kidney cells manifested significantly less tissue factor procoagulant activity than their disrupted counterparts. Addition of calcium ionophore A23187 rapidly and reversibly enhanced the cell-surface expression of tissue factor in intact cells up to the level achieved by disruption. Inhibitors of calmodulin blocked the ionophore-dependent enhancement of...

Regulation of monocyte procoagulant activity in acute myocardial infarction: role of tissue factor and tissue factor pathway inhibitor-1.

In acute myocardial infarction (AMI), monocyte procoagulant activity is increased and may contribute to the risk for recurrence and other thrombotic events. This study sought to investigate the role tissue factor (TF) and tissue factor pathway inhibitor-1 (TFPI-1) in the regulation of monocyte procoagulant activity in AMI. Serial venous blood samples were obtained from 40 patients with AMI unde...

Destruction of factor VIII procoagulant activity in tissue culture media.

Human endothelial cells have been repoded to synthesize protein that has von Willebrand factor activity and reacts with a heterologous antibody to plasma factor VIll/von Willebrand factor ( FVlll/vWF); however, no FVlll procoagulant activity has been associated with the protein produced by endothelial cells. Of its three biologic properties, only the FVlll procoagulant activity of purified plas...

GLucocorticoids inhibit the generation of leukocyte procoagulant (tissue factor) activity.

Kinetics of lung tissue factor expression and procoagulant activity in bleomycin induced acute lung injury

BACKGROUND Activation of coagulation by expression of tissue factor (TF) in the airspace is a hallmark of acute lung injury (ALI) but the timing of TF activation in relationship to increases in lung permeability and inflammation are unknown. METHODS To test the hypothesis that TF is upregulated early in the course of acute bleomycin lung injury and precedes increased permeability and inflamma...