Expression of the MexXY efflux pump in amikacin-resistant isolates of Pseudomonas aeruginosa

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Investigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit

Background and Aims: Pseudomonas aeruginosa is one of the most important pathogens of nosocomial infections, especially in the ICU (Intensive Care Unit), which has resistance to a wide range of antibiotics, especially Carbapenems. Among the most important resistance mechanisms of this bacteria against carbapenems are MexAB-OprM efflux pump. Therefore, the aim of this study was to evaluate the g...

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MexXY multidrug efflux system of Pseudomonas aeruginosa

Anti-pseudomonas aminoglycosides, such as amikacin and tobramycin, are used in the treatment of Pseudomonas aeruginosa infections. However, their use is linked to the development of resistance. During the last decade, the MexXY multidrug efflux system has been comprehensively studied, and numerous reports of laboratory and clinical isolates have been published. This system has been increasingly...

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Expression in Escherichia coli of a new multidrug efflux pump, MexXY, from Pseudomonas aeruginosa.

Two new genes (mexXY) similar to mexAB, mexCD, and mexEF and mediating multidrug resistance were cloned from the chromosome of Pseudomonas aeruginosa. Elevated ethidium extrusion was observed with Escherichia coli cells harboring the plasmid carrying mexXY. This MexXY system confers higher resistance to fluoroquinolones than the MexAB and MexCD systems, and E. coli ToIC or P. aeruginosa OprM is...

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Synergistic Effect of Silver Nanoparticles and Streptomycin Antibiotic on the MexX Gene Expression of Pump Efflux System in Drug-Resistant Pseudomonas aeruginosa Strains

Introduction: Pseudomonas aeruginosa is one of the most important infectious agents in humans, which is difficult to control in hospitals due to its resistance to various antibiotics. Efflux pump systems play an important role in the drug resistance of this bacterium to a variety of antibiotics. This study aimed to determine the antimicrobial synergistic effect of silver nanoparticles and the a...

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MexXY-OprM efflux pump is necessary for a adaptive resistance of Pseudomonas aeruginosa to aminoglycosides.

Exposure of Pseudomonas aeruginosa to aminoglycosides frequently selects for recalcitrant subpopulations exhibiting an unstable, "adaptive" resistance to these antibiotics. In this study, we investigated the implication in the phenomenon of MexXY-OprM, an active efflux system known to export aminoglycosides in P. aeruginosa. Immunoblotting experiments demonstrated that the transporter MexY, but...

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ژورنال

عنوان ژورنال: Clinical Microbiology and Infection

سال: 2004

ISSN: 1198-743X

DOI: 10.1111/j.1469-0691.2004.00991.x