Expression of ssDNA in Mammalian Cells

Expression of ssDNA in mammalian cells.

Antisense therapy involves the use of antisense oligonucleotides for altering targeted gene function. However, the low efficiency of cell delivery of antisense oligonucleotides has limited the efficacy of antisense therapeutic approaches. RNA-based antisense or ribozyme oligonucleotides can be either synthesized endogenously (e.g., by a viral vector) or delivered exogenously. However, there is ...

Expression of Biologically Active Recombinant B-Domain-Deleted Human Factor VIII in Mammalian Cells

Expression of Egasyn - Esterase in Mammalian Cells

Mouse egasyn cDNA was inserted into expression vector pCDpoly and transfected into mammalian cell lines. Transfected human HepG2 cells, monkey COS-1 cells, and mouse L cells expressed egasyn-esterase catalytic activity. Within COS-1 cells, egasyn was localized to the endoplasmic reticulum. Although individual cells produced large amounts of egasyn, no secretion was observed. No 8-glucuronidase-...

expression of biologically active recombinant b-domain-deleted human factor viii in mammalian cells

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Expression in mammalian cells using BacMam viruses

The baculovirus expression vector system has been commonly employed as a rapid and inexpensive approach for recombinant protein production in insect cells (1, 2). Despite the wide applications, baculovirus infection results in insect cell death and lysis in a few days post-infection, which can lead to suboptimal protein processing late in the infection phase. In addition, some proteins that are...