Expression of gene 1.2 and gene 10 of bacteriophage T7 is lethal to F plasmid-containing Escherichia coli
نویسندگان
چکیده
منابع مشابه
Frameshifting in gene 10 of bacteriophage T7.
Gene 10 of bacteriophage T7, which encodes the most abundant capsid protein, has two products: a major product, 10A (36 kDa), and a minor product, 10B (41 kDa). 10B is produced by frameshifting into the -1 frame near the end of the 10A coding frame and is incorporated into the capsid. The frameshift occurs at a frequency of about 10% and is conserved in bacteriophage T3. This study shows that s...
متن کاملDefective transcription of the right end of bacteriophage T7 DNA during an abortive infection of F plasmid-containing Escherichia coli.
Transcription of T7 and mutant T3 DNA during infections of F plasmid-containing cells has been analyzed by using Southern hybridization. A transcriptional defect is apparent in these abortively infected cells that is most severe in the class III region of the phage genome. In particular, RNAs that are initiated from the gene 13 promoter are not elongated to give full-length molecules. It is sug...
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متن کاملRecombinants between bacteriophages T7 and T3 which productively infect F-plasmid-containing strains of Escherichia coli.
Recombinant phages between T7 and T3 have been isolated that grow well on strains of Escherichia coli that contain the F factor. One phage that has been characterized physically and genetically is predominately of the T7 genotype. Within this hybrid phage, two separate regions of T3 DNA have been located which are necessary for the phenotype of productive growth on F-containing strains. One of ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1991
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.173.4.1536-1543.1991