Expression of Furfural Reductase Improved Furfural Tolerance in Antarctic Bacterium Pseudomonas extremaustralis

Whole-cell biocatalysis using Antarctic bacteria is presently hampered by a lack of genetic information, limited gene tools and critically, poor range cultivation conditions. In this work, biological engineering strategy was employed for developing Pseudomonas extremaustralis, metabolically-versatile biopolymer-producing bacterium, as new whole-cell biocatalytic host. For purpose, cloning plasmid construction were carried out overexpression furfural reductase (FucO), an industrially-important enzyme degradation toxic compound commonly found in lignocellulosic biorefinery. FucO from Escherichia coli BL21 cloned pJM105 transformed into competent cells P. extremaustralis to generate biologically-engineered pFucO strain. functional characterization the enzyme, activity assayed, where strain exhibited increased about 15.6 U/mg, 18.8-fold higher than empty plasmid-carrying control (0.83 U/mg). Furfural detoxification whole also determined which led tolerance cell growth with OD600 value 5.3 compared that inhibited 10 mM during 48-hour cultivation. Therefore, findings obtained study successfully demonstrated development host production recombinant reductase. The bioengineering would serve modular biotechnological platform polar bioproduct tailored towards industrial biotechnology applications.