Expression of factor VIII-related antigen in human aqueous drainage channels

Localization of factor-VIII-related antigen in human vascular subendothelium.

Factor-VIII-related antigen has previously been shown to be synthesized by vascular endothelial cells. Using both an immunofluorescent staining technique and electron microscopy, we have demonstrated the presence of factor-VIII-related antigen in human vascular subendothelium. This finding may have implications in the mechanism of platelet adhesion to deendothelialized blood vessel surfaces.

Factor-VIII-related antigen: multiple molecular forms in human plasma.

Analysis of Factor-VIII-related antigen (vWAg) in normal plasma by crossed immunoelectrophoresis reveals an asymmetric pattern indicative of molecular heterogeneity. This asymmetric pattern apparently results from the presence of multiple molecular forms of VWAg with distinct, though partially overlapping, mobilities on crossed immunoelectrophoresis. Evidence for this conclusion has been obtain...

Electrophoretic studies of human factor VIII-related antigen [proceedings].

I I the inherent lytic rate of cross-linked and non-cross-linked clots. To investigate this, a fibrin-plate techique was used in which small equal volumes of haemoglobin solution were spotted on to the plates instead ofthestreptokinase solution,and therateof diffusion of the haemoglobin into the plates was estimated. Yet again this was unaffected by crosslinking, and, since streptokinase has an...

Expression of Biologically Active Recombinant B-Domain-Deleted Human Factor VIII in Mammalian Cells

Bacterial Expression and Purification of C1C2 Domain of Human Factor VIII

With the aim of the production of human factor VIII antigen and its corresponding antibody an epitope coding fragment of the light-chain of hFVIII, fused to a His6-tag, was isolated and over-expressed in Escherichia coli. The over-expressed hFVIII-epitope containing peptide was confirmed by its reaction with a rabbit serum directed against native hFVIII as well as antiHis6-tag antibody. An expr...