Expression of extracellular superoxide dismutase in the human male reproductive tract, detected using antisera raised against a recombinant protein

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Expression of extracellular superoxide dismutase in the human male reproductive tract, detected using antisera raised against a recombinant protein.

Mammalian spermatozoa are particularly susceptible to the deleterious effects of reactive oxygen species and lipid peroxidation, which ultimately lead to impaired fertility. A number of enzymes are present in the male reproductive tract which may play a role in preventing oxidative damage; in particular, the epididymis is the site of synthesis and secretion of large amounts of extracellular sup...

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Background: Male infertility refers to the inability of a male to achieve a pregnancy in a fertile female. The root of many causes of infertility is miscommunication between immune and reproductive system. Male reproductive system is very sensitive and vulnerable, infections can hinder maturation and movement of spermatozoa lead to impaired fertility.All species need an immediate reply to the m...

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Evaluating the Effect of Protein A Signal Peptide on Extracellular Expression of Recombinant Hirudin in E.Coli

Abstract         Background and Objective: Hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. Recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. The aim of this study was to evaluate the effect of inserting protein A signal peptide sequence of pEZZ18 plasmid on expression and secretion...

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Expression of Extracellular Superoxide Dismutase Protein in Diabetes

BACKGROUND Diabetes is characterized by chronic hyperglycemia, which can increase reactive oxygen species (ROS) production by the mitochondrial electron transport chain. The formation of ROS induces oxidative stress and activates oxidative damage-inducing genes in cells. No research has been published on oxidative damage-related extracellular superoxide dismutase (EC-SOD) protein levels in huma...

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ژورنال

عنوان ژورنال: Molecular Human Reproduction

سال: 1998

ISSN: 1460-2407

DOI: 10.1093/molehr/4.3.235