Expression and characterization of virus-like particles containing rubella virus structural proteins

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Expression and characterization of virus-like particles containing rubella virus structural proteins.

Rubella virus (RV) virions contain two envelope glycoproteins (E1 and E2) and a capsid protein (C). Noninfectious RV-like particles (VLPs) containing three structural proteins were expressed in a BHK cell line (BHK-24S) by using an inducible promoter. These VLPs were found to resemble RV virons in terms of their size, their morphology, and some biological activities. In immunoblotting studies, ...

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5 ACKNOWLEDGEMENTS 6 ORIGINAL PUBLICATIONS 7 ABBREVIATIONS 8

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In order to increase our understanding of the assembly of the capsid proteins of AHSV, for the development of subunit vaccines and to study viral morphogenesis, it is necessary to investigate the interaction of the four major structural proteins of AHSV in the absence of other AHSV proteins and the viral genome. To investigate particle formation it was necessary to synthesise the proteins of in...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1994

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.68.6.4086-4091.1994