Expression analysis of a Cucurbita cDNA encoding endonuclease.
نویسندگان
چکیده
منابع مشابه
Molecular cloning and expression of a cDNA encoding an apoptotic endonuclease DNase gamma.
An endonuclease named DNase gamma has been purified from the nuclei of apoptotic rat thymocytes [Shiokawa, Ohyama, Yamada and Tanuma (1997) Biochem. J. 326, 675-681]. Here we report the molecular cloning of a cDNA encoding a 35 kDa precursor protein for rat DNase gamma. A 1.6 kb mRNA coding for the DNase gamma precursor is detected at high levels in spleen, lymph nodes, thymus and liver. By usi...
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Granulins are a group of highly conserved growth factors that have been described from variety of some organisms spanning the metazoan. It has been shown to have roles in multiple processes involved in cell growth, development inflammation, wound healing and tissue remodeling. In the present study, we isolated the full-length cDNA encoding Nile tilapia granulin gene by in silico cloning and 5iR...
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Poly(A) RNA was isolated from a rat medullary thyroid carcinoma that exhibited high levels of immunoreactive cholecystokinin (CCK). Double-stranded cDNA was synthesized from the poly(A) RNA and inserted into the Pst I site of pBR322. Bacterial colonies containing CCK cDNA were identified using the hybridization probe d(T-C-C-A-T-C-C-A-N-C-C-C-A-T-G-T-A-G-T-C). The sequence of the probe was dedu...
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15 صفحه اولIsolation and Characterization of Expression cDNA Clones Encoding Antigens
The isolation of recombinant cDNA clones expressing antigens found in Onchocerca volvulus infective larvae is described. To isolate such clones, an expression cDNA library constructed from adult 0. volvulus RNA was screened with antiserum raised against infective larvae. One clone, designated XRAL-1 was characterized further. The recombinant antigen produced by XRAL-1 stimulates proliferation o...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 1995
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.1995_4643