Exposure time in scintillation autoradiography of 3H-incorporated samples.
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چکیده
منابع مشابه
[3H]ouabain autoradiography of frog retina
The kinetics and distribution of ouabain binding in retinas of Rana pipiens were examined quantitatively by scintillation counting and freeze-dry autoradiography. The time-course of binding at several concentrations was consistent with a bimolecular reaction. Estimated equilibrium binding levels gave a Michaelis-Menton relationship with a Km = 8.3 x 10(-8) M and a maximum binding level (Bmax) =...
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A technique has been developed to investigate [3H]sulpiride binding in rat brain sections using quantitative autoradiography and tritium-sensitive film. Binding was saturable and reversible with very low nonspecific binding. [3H]Sulpiride bound to an apparent single population of sites in striatum with a Kd of 3.2 nM and Bmax of 447 fmol/mg protein. Binding sites were localized in the lamina gl...
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We have used [3H]2-deoxyglucose autoradiography to correlate the labeling of individual neurons with electrical activity within the central nervous system of a terrestrial mollusc, Limax maximus. In an electrically quiescent control preparation where a single neuron is impaled with a glass microelectrode but not stimulated, several somata are uniformly labeled at 3-5 times background. In prepar...
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ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1984
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.33.8_547