Exploring Clinacanthus nutans leaf different solvent extracts on antiproliferative effects induced metastasis through apoptosis and cell cycle against MCF-7 human breast cancer cell lines
نویسندگان
چکیده
Abstract Background Medicinal herbs in Malaysia like Clinacanthus nutans (CN) traditionally are used the treatment of various diseases and cancers. The present research was conducted to determine effects C. leaf different solvent extracts on human breast cancer cell lines (MCF-7). antiproliferative growth survival dichloromethane CN (CNDCM), as well short- long-term through metastasis, apoptosis cycle effects, were observed. chemical profiles done evaluate properties CNDCM. Results evaluation GC–MS identified 16 major phytochemical compounds this extract with biological activities. Antiproliferative assay is SRB assay, which showed CNDCM induced strong property compared other extracts, so its IC 50 dose selected for further testing value 108 µg/mL at 72 h after exposure MCF-7 MCF-10A lines. clonogenic concentrations (31.25, 62.5, 125, 250 500 µg/mL) inhibited ability cells form colonies, metastasis result indicated an image wound healing assay. Moreover, significantly all phases. Finally, experiments normal no tested. Conclusion Among CN, demonstrated highest activity against
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ژورنال
عنوان ژورنال: Future Journal of Pharmaceutical Sciences
سال: 2022
ISSN: ['2314-7245', '2314-7253']
DOI: https://doi.org/10.1186/s43094-022-00437-1