Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping

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Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping

The Festuca genus is thought to be the most numerous genus of the Poaceae family. One of the most agronomically important forage grasses, Festuca pratensis Huds. is treated as a model plant to study the molecular mechanisms associated with tolerance to winter stresses, including frost. However, the precise mapping of the genes governing stress tolerance in this species is difficult as its karyo...

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Physical and genetic mapping in the grasses Lolium perenne and Festuca pratensis.

A single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line 2n = 2x = 14. In this line recombination occurs throughout the length of the F. pratensis/L. perenne bivalent. The F. pratensis chromosome and recombinants between it and its L. perenne homeologue can be visualized using genomic in situ hybridizat...

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Leaf Senescence in a Nonyellowing Mutant of Festuca pratensis: Metabolism of Cytochrome f.

In a mutant genotype of Festuca pratensis Huds., net degradation of a number of thylakoid membrane proteins during senescence is impaired. Previous studies have suggested that the highly hydrophobic intrinsic chlorophyll-binding proteins were the definitive subjects of the metabolic lesion. In the present study we find that cytochrome f, as determined by haem-staining, Western blotting, enzyme-...

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AIM Pig liver carboxylesterase (PLE) gene sequences in GenBank are incomplete, which has led to difficulties in studying the genetic structure and regulation mechanisms of gene expression of PLE family genes. The aim of this study was to obtain and analysis of complete gene sequences of PLE family by screening from a Rongchang pig BAC library and third-generation PacBio gene sequencing. METHO...

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ژورنال

عنوان ژورنال: PLOS ONE

سال: 2017

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0179043