Explaining differences in saturation levels for Affymetrix GeneChip® arrays
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چکیده
منابع مشابه
Explaining differences in saturation levels for Affymetrix GeneChip® arrays
The experimental spike-in studies of microarray hybridization conducted by Affymetrix demonstrate a nonlinear response of fluorescence intensity signal to target concentration. Several theoretical models have been put forward to explain these data. It was shown that the Langmuir adsorption isotherm recapitulates a general trend of signal response to concentration. However, this model fails to e...
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Large-scale microarray expression profiling studies have helped us to understand basic biological processes and to classify and predict the prognosis of cancers; they have also accelerated the identification of new drug targets. Affymetrix GeneChip probe arrays are high-density oligonucleotide microarrays that are available for many prokaryotic and eukaryotic species. Affymetrix human and mouse...
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■ Abdueva D., et al. Experimental Comparison and Evaluation of the Affymetrix Exon and U133 Plus 2.0 GeneChip Arrays. PLoS ONE 2(9):e913 (2007). Key Findings: ▪ Abdueva et al. compared the performance of GeneChip Exon Arrays and Human Genome U133 Plus 2.0 Arrays through a series of spike-in hybridization experiments and demonstrated high comparability between the two arrays. ▪ “Despite several ...
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Affymetrix Genechip microarrays are used widely to determine the simultaneous expression of genes in a given biological paradigm. Probes on the Genechip array are atomic entities which by definition are randomly distributed across the array and in turn govern the gene expression. In the present study, we make several interesting observations. We show that there is considerable correlation betwe...
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MOTIVATION The defining feature of oligonucleotide expression arrays is the use of several probes to assay each targeted transcript. This is a bonanza for the statistical geneticist, who can create probeset summaries with specific characteristics. There are now several methods available for summarizing probe level data from the popular Affymetrix GeneChips, but it is difficult to identify the b...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2007
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkm348