Expired Platelet Concentrate as a Source of Human Platelet Lysate for Xenogeneic-Free Culture of Human Dermal Fibroblasts

Dermal fibroblasts have been used clinically to promote wound healing and reduce wrinkles. Most of the time, fetal bovine serum (FBS) is for expansion fibroblasts. In addition, chemically defined medium can also be fibroblast expansion. Nonetheless, both FBS are not ideal culture cells that will as has risk pathogen transmission induction xenogeneic immune response whilst extremely expensive. this study, we examine potential using human platelet lysate (hPL) prepared from expired concentrates dermal For experiments, were cultured with 5 10% hPL, control group compare cell morphology, viability, growth rate, extracellular matrix gene expression healing. Results showed hPL more elongated smaller in size. The viability was higher than 90% all groups. Expansion significantly shorten population doubling time compared 5% However, lower type I collagen, III collagen fibronection well slower closure. summary, a substitute expand it increases proliferation. further studies required determine if changes ECM migration hPL-expanded affect efficacy promoting vivo