Expanding the genetic code of Mus musculus

Expanding the genetic code of Mus musculus

Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Nɛ-acetyl-lysine. Stable integration of transgenes encoding an engineered Nɛ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal...

Mus musculus : Genetic Portrait of the House Mouse Reference

role in the study of genetics ever since Carl Correns, Hugo De Vries, and Erich Von Tschermak independently rediscovered Mendel’s laws at the beginning of the twentieth century. Because these three scientists, as well as Mendel himself, performed their research entirely on plants, many in the scientific community questioned whether Mendel’s laws could explain the basis for inheritance in animal...

Expanding the genetic code.

Although chemists can synthesize virtually any small organic molecule, our ability to rationally manipulate the structures of proteins is quite limited, despite their involvement in virtually every life process. For most proteins, modifications are largely restricted to substitutions among the common 20 amino acids. Herein we describe recent advances that make it possible to add new building bl...

Genetic control of a serum esterase component in Mus musculus.

Genetic control of erythrocytic esterase forms in Mus musculus.

TARCH or agar gel electrophoresis coupled with azo dye staining methods has permitted the detection of genetically determined variants of several forms of nonspecific carboxylic esterases in a protozoan (ALLEN 1961), maize ( SCHWARTZ 1960, 1964), Drosophila (WRIGHT 1963; WRIGHT and MACINTYRE 1963), and house flies (VELTHUIS and VAN ASPEREN 1963). In mammals, inherited electrophoretic variants o...