Ex vivo cytokine release reflects sensitivity to occupational endotoxin exposure

Ex vivo cytokine release reflects sensitivity to occupational endotoxin exposure.

Not everyone exposed to endotoxin develops respiratory symptoms, even at very high exposure levels. The aim was to investigate whether ex vivo lipopolysaccharide (LPS)-induced cytokine release may be predictive of individual sensitivity to occupational endotoxin exposure. In 412 agricultural workers, tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-10 release was measured in su...

Occupational exposure to endotoxin from contaminated dental unit waterlines.

INTRODUCTION Fine aerosolised droplets and splatter generated during dental procedures influences the composition of the indoor air quality of dental care facilities and poses a potential threat to the workers' health. Therefore there is a need to assess the risks from exposure to bioaerosols and splatter and identify measures of reducing possible hazards. METHODOLOGY A review of the literatu...

Reduced ex vivo chemokine production by polymorphonuclear cells after in vivo exposure of normal humans to endotoxin.

Monocytes from patients with sepsis have a reduced capacity to produce cytokines, a state referred to as immunoparalysis. To determine whether polymorphonuclear leukocytes (PMNL) can be rendered hyporesponsive, PMNL from 6 healthy volunteers intravenously challenged with lipopolysaccharide (LPS; 4 ng/kg) were stimulated ex vivo with heat-killed bacteria or LPS, and the release of the CXC chemok...

Endotoxin receptor site. II. Specificity of endotoxin receptor of platelets and sensitivity to endotoxin in vivo.

The biological specificity of the endotoxin receptor on platelet membranes was examined. The binding indices of platelets in experimental endotoxemia which was induced by intravenous administration of endotoxin (Lipopolysaccharide of E. coli, Difco) to rabbits were found to be 30% of the control at 60 min after the injection. The result suggests that the endotoxin receptor of platelets was alre...

Ex Vivo Cytokine and Memory T Cell