Evidence That the Thyrotropin Receptor Ectodomain Contains Not One, But Two, Cleavage Sites*
نویسندگان
چکیده
منابع مشابه
Folding-dependent binding of thyrotropin (TSH) and TSH receptor autoantibodies to the murine TSH receptor ectodomain.
The mouse TSH receptor ectodomain (mTSHR-ecd) was amplified from murine thyroid complementary DNA and ligated into the pAcGP67B insect cell vector, and the nucleotide sequence was confirmed. Employing a baculovirus-insect cell system, the mTSHR-ecd (amino acids 22-415) was expressed as a fusion protein with the gp67 insect cell signal sequence at the NH2-terminus and a C-terminal six-histidine ...
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Gangliosides were extracted from purified human and porcine thyrotropin (TSH) receptors (TSH-R) and were detected by probing with an 125I-labeled sialic acid-specific lectin, Limax flavus agglutinin. Gangliosides copurified with human and porcine TSH-R migrated between monosialoganglioside GM1 and disialoganglioside GD1a. Ceramide glycanase digestion of the purified human TSH-R-associated glyco...
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Dipartimento di Biologia, Universita di Padova, Via Trieste 75, 351 21 Padova, ltaly (L.N., B.B., P.M.); lnstitut für Biochemie III, Eldgenossische Technische Hochschule Zentrum, Universitatsstrasse 16, CH-8092 Zürich, Switzerland (P.D., P.J.); and National Research Council Unit for Muscle Biology and Physiopathology, Dipartimento di Scienze Biomediche Sperimentali, Universita di Padova, Via Tr...
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ژورنال
عنوان ژورنال: Endocrinology
سال: 1997
ISSN: 0013-7227,1945-7170
DOI: 10.1210/endo.138.7.5259