Evidence of Phenylalanine Transaminase Activity in the Isoenzymes of Aspartate Transaminase

Evidence of phenylalanine transaminase activity in the isoenzymes of aspartate transaminase.

The transamination by pig mitochondrial and supernatant aspartate transaminase of aromatic amino acids was studied in order to determine if an analogy exists between these enzymes and a previously reported brain phenylalanine transaminase (GEORGE, H., AND GABAY, S. (1968) Biochim. Biophys. Ada, 167, 555). Supernatant and mitochondrial aspartate transaminase from pig heart can utilize aromatic a...

Urinary aspartate transaminase in childhood.

Dffluoro-oxaloacetate with Aspartate Transaminase

Difluoro-oxaloacetate interacts with the aldimine form of aspartate transaminase to give a complex, the dissociation constant ofwhich has been determined spectrophotometrically and by 19F n.m.r. (nuclear magnetic resonance). The 19F n.m.r. line-width-pH and chemical-shift-pH profiles of difluoro-oxaloacetate in the presence of the aldimine form of the enzyme both show inflexion points in the pH...

Differential scanning calorimetry of cytoplasmic aspartate transaminase.

Differential scanning calorimetry has been applied to study factors affecting the thermally induced denaturation of cytoplasmic aspartate aminotransferase, a dimeric pyridoxal enzyme. The consequences of binding of coenzyme and substrate derivatives to both the apo and holo forms of the enzyme were investigated and are interpreted in terms of the stabilization of the native form of the enzyme. ...

Gamma-glutamyltransferase, alanine transaminase and aspartate transaminase levels and the diagnosis of gestational diabetes mellitus.

OBJECTIVES To evaluate gamma-glutamyltransferase (GGT), alanine transaminases (ALT) and aspartate transaminases (AST) levels and prevalent gestational diabetes mellitus (GDM). DESIGN AND METHODS Random plasma glucose, GGT, ALT and AST and the 50-g glucose challenge test were done on antenatal women followed by diagnostic 3-point 75-g oral glucose tolerance test within two weeks. GDM was diagn...