Evidence for non-enzymatic glycosylation ofEscherichia colichromosomal DNA
نویسندگان
چکیده
منابع مشابه
Evidence for non-enzymatic glycosylation of Escherichia coli chromosomal DNA.
We have recently shown that the process of non-enzymatic glycosylation (glycation) takes place in Escherichia coli under physiological conditions and affects both recombinant and endogenous bacterial proteins. In this study, we further demonstrate that E. coli chromosomal DNA is also subjected to glycation under physiological growth conditions. The E. coli DNA accumulates early glycation (Amado...
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The success of oligonucleotide ligation assays in probing specific sequences of DNA arises in large part from high enzymatic selectivity against base mismatches at the ligation junction. We describe here a study of the effect of mismatches on a new non-enzymatic, reagent-free method for ligation of oligonucleotides. In this approach, two oligonucleotides bound at adjacent sites on a complementa...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2005
ISSN: 0950-382X
DOI: 10.1111/j.1365-2958.2005.04504.x