Evaluation of whole-genome sequencing as a genotyping tool for Campylobacter jejuni in comparison with pulsed-field gel electrophoresis and flaA typing
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چکیده
منابع مشابه
Evaluation of whole-genome sequencing as a genotyping tool for Campylobacter jejuni in comparison with pulsed-field gel electrophoresis and flaA typing.
Campylobacter jejuni is a leading cause of foodborne illness, with poultry and poultry products being leading sources of infection. Epidemiological efforts to trace Campylobacter can be challenging because of the extreme genetic diversity of this bacterium relative to other foodborne pathogens. To enhance tracking and epidemiological efforts, whole-genome sequencing has been used for other food...
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A total of 100 Campylobacter coli isolates from swine reared in conventional and antimicrobial free (ABF) farms were genotyped using multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) to evaluate their discriminatory power, throughput and index of association to determine the genotypic similarity and clonal structure of the isolates. MLST had a higher discriminatory p...
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BACKGROUND Increased lipoprotein(a) is a risk factor for atherosclerosis, and its concentration in serum is inversely correlated with the size of the apoliprotein(a) [apo(a)] component. The size of the apo(a) gene is determined mainly by the Kringle IV size polymorphism. We have optimized and characterized pulsed field gel electrophoresis (PFGE) for apo(a) genotyping. METHODS Established PFGE...
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We developed a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Campylobacter isolates based on the standardized protocols used by PulseNet laboratories for the subtyping of other food-borne bacterial pathogens. Various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentrat...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 2013
ISSN: 0032-5791
DOI: 10.3382/ps.2012-02695