Evaluation of ram semen motility by a swim-up technique
نویسندگان
چکیده
منابع مشابه
Sperm swim-up: a simple and effective technique of semen processing for intrauterine insemination.
OBJECTIVE To study the impact of sperm swim up technique of seminal processing for the purpose of intrauterine insemination (IUI) in couples presenting with subfertility. METHODS Hundred and twenty-one couples presenting with subfertility underwent 281 cycles of IUI in Combined Military Hospital Kharian, Lahore and PNS SHIFA Karachi from June 2002 to March 2005. Men had prior semen analysis t...
متن کاملSemen preparation by standard swim-up versus swim-up with test yolk buffer incubation in intrauterine insemination: a randomized study.
In order to compare the standard swim-up semen preparation with and without test yolk buffer (TYB) incubation in intrauterine insemination (IUI), we conducted a prospective multicentre randomized trial. A total of 121 infertile couples with male factor (n = 52) or unexplained infertility (n = 69) was randomly assigned to two groups following ovulation induction. Semen was prepared by standard s...
متن کاملP-139: Ram Sperm Motility Following 48 Hours of Refrigeration: Evaluation of Different Extenders for The Preservation of Ram Semen in Liquid State
Background: The application of laparoscopic method for intrauterine insemination is limited in sheep industry by the high cost of equipment and low pregnancy rate of frozen-thawed spermatozoa. Both disadvantages could be decreased if chilled ram semen is transported to the farms and inseminated through cervix. Semen extender provides the requirements for sperm and affects its motility, viabilit...
متن کاملStorage of ram semen.
Storage of ram semen in liquid and frozen state, the diluents used for both methods, processing, cooling, freezing and thawing of semen are reviewed. Factors influencing the fertility of stored semen and methods used for improvement are discussed, and fertility results of long-term frozen stored ram semen are also given.
متن کاملEvaluation of the semen swim-up method for bovine sperm RNA extraction.
Isolation of high-quality RNA is important for assessing sperm gene expression, and semen purification methods may affect the integrity of the isolated RNA. This study evaluated the effectiveness of the sperm swim-up method for seminal RNA isolation. Frozen semen samples in straws from three bulls of proven fertility were purified by the swim-up method. RNA extraction was carried out using the ...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1993
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0970339