Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus
نویسندگان
چکیده
منابع مشابه
Factors Affecting Agrobacterium-mediated Transformation of Plants
Plant transformation technology has become a versatile platform for cultivar improvement as well as for studying gene function in plants. The development of an efficient method for genetic transformation is a prerequisite for the application of molecular biology to the improvement of a given crop species. Agrobacterium-mediated genetic transformation is the dominant technology used for the prod...
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We describe a streamlined and systematic method for cloning green fluorescent protein (GFP)-open reading frame (ORF) fusions and assessing their subcellular localization in Arabidopsis thaliana cells. The sequencing of the Arabidopsis genome has made it feasible to undertake genome-based approaches to determine the function of each protein and define its subcellular localization. This is an ess...
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ژورنال
عنوان ژورنال: Journal of Integrative Agriculture
سال: 2017
ISSN: 2095-3119
DOI: 10.1016/s2095-3119(16)61460-0