Evaluation of Membrane Integrity of Canine Epididymal Spermatozoa by Short Hypoosmotic Swelling Test with Ultrapure Water
نویسندگان
چکیده
منابع مشابه
Determination of membrane integrity of canine spermatozoa
Semen samples from 9 sexually mature dogs of different breeds, aged from 1 to 5 years were used. Quality of sperm was evaluated using the following parameters: progressive motility (subjective), then structural and functional integrity of spermal membrane (supravital colouring (eosine/nigrosine) and HOS test). The majority of HOS reactive or swollen spermatozoa was placed in a hypoosmolar solut...
متن کاملLow-density Lipoprotein Improves Motility and Plasma Membrane Integrity of Cryopreserved Canine Epididymal Spermatozoa.
Cryopreservation of caudal epididymal spermatozoa is an effective technique to conserve genetic potentials of superior dogs when it is not possible to collect ejaculated spermatozoa. Although hen egg yolk is commonly supplemented into the semen extender, active substances within the egg yolk which protect sperm against cryoinjury remain to be discovered. Among its compositions, low-density lipo...
متن کاملWater-induced hypo-osmotic test for the evaluation of canine sperm membrane integrity
The purpose of this study was to investigate the efficiency of the water test to evaluate the functional membrane integrity of ejaculated canine sperm after collecting, cooling, and freezing-thawing. Twenty-five ejaculates were obtained from 12 stud dogs by digital manipulation. Sperm-rich fractions were evaluated, diluted, cooled at 5oC and frozen-thawed. A conventional hypo-osmotic swelling t...
متن کاملHypoosmotic Sperm Swelling Test: Reevaluation of Its Potential Use
The aim of this study was to assess the predictive value of the hypoosmotic sperm swelling test. Good correlations were observed between the percentage of spermatozoa undergoing swelling and the percentage motility both in normal (r = 0.68; p < 0.001) and infertile patients (r = 0.65; p < 0.001). In contrast, sperm swelling did not significandy correlate either with sperm oocyte fusion (assesse...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2003
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.65.817