Evaluation of IGF-1, TNF-α, and TGF-β Gene Expression after Oral Vitamin D Supplementation in School-Aged Children with Chronic Bronchial Asthma

BACKGROUND: Airway remodeling in children with bronchial asthma is due to the effect of inflammatory mediators and growth factors on epithelium. Vitamin D (VitD) has immunomodulatory many diseases as asthma. The ant-inflammatory anti-fibrotic role VitD could prevent or improve air way asthmatic patients. AIM: study investigated supplementation expression transforming factor-beta (TGF-β), tumor necrosis factor-alpha (TNF-α), insulin factor 1(IGF-1) correlate them severity level control. METHODS: serum mRNA IGF-1, TGF-β, TNF-α were estimated 50 patients 20 healthy controls control subjects using quantitative PCR real-time. Asthmatic deficiency received for 2 months followed by remeasurement genes TNF-α, IGF-1. RESULT: Pre-intake lower all than (p = 0.005). was directly correlated IGF-1 expression, which indirectly r 0.5 −0.57; p 0.0001 0.002, respectively. After supplementation, TGF-β gene only that decreased significantly 0.04) together improved spirometric parameters. CONCLUSIONS: down regulated level, but it did not affect