Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida

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Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida.

This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The resu...

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Superoxide dismutase and catalase activities in Photobacterium damselae ssp. piscicida.

The ability of a set of Photobacterium damselae ssp. piscicida strains isolated from different fish species to produce different superoxide dismutase (SOD) and catalase enzymes was determined. Unlike other bacterial pathogens, P. damselae ssp. piscicida is not able to produce different isoforms of SOD or catalase containing different metal cofactors when cultured under oxidative stress induced ...

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Simple and rapid detection of Photobacterium damselae ssp. piscicida by a PCR technique and plating method.

AIMS To detect Photobacterium damselae ssp. piscicida using the PCR technique and plating method. METHODS AND RESULTS Two strains of P. damselae ssp. piscicida were isolated from cultured cobia (Rachycentron canadum) at two different fish farms in Taiwan. A pair of primers was designed to detect the capsular polysaccharide gene of P. damselae ssp. piscicida by PCR. Reference strains of differ...

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Phenotypic characterization of the marine pathogen Photobacterium damselae subsp. piscicida.

The taxonomic position of Photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis, is controversial as this organism has also been described as 'Pasteurella piscicida'. To clarify the taxonomic position of the pathogen, a total of 113 P. damselae subsp. piscicida strains and 20 P. damselae subsp. damselae strains, isolated from different geographical areas and from ...

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Survival against exogenous hydrogen peroxide of Photobacterium damselae subsp. piscicida under different culture conditions.

Photobacterium damselae subsp. piscicida is a fish pathogen responsible for important losses in aquaculture world-wide. Several studies on its virulence mechanisms have been carried out and outer membrane proteins involved in the acquisition of iron or production of extracellular products have been suggested as the main determinants of its virulence for fish (Magariños, Santos, Romalde, Rivas, ...

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ژورنال

عنوان ژورنال: Biological Research

سال: 2007

ISSN: 0716-9760

DOI: 10.4067/s0716-97602007000100009