Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR
نویسندگان
چکیده
منابع مشابه
Selection of suitable reference genes for real-time PCR studies of early developmental stages of sturgeons
In quantitative real-time PCR, the mRNA level can be quantified in relative terms based on the expression ratio of mRNAs of the target gene and an internal reference gene. Since, an internal standard should be expressed at a constant level among different tissues of an organism at all stages of development, and should be unaffected by the experimental treatment, the stability of different refer...
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ژورنال
عنوان ژورنال: Molecular Breeding
سال: 2009
ISSN: 1380-3743,1572-9788
DOI: 10.1007/s11032-009-9259-x