Evaluation of Biofilm formation capacity of Pasteurella multocida and its relationship with antibiotic resistance

Pasteurella multocida is a Gram-negative bacterium that causes haemorrhagic septicaemia in cattle and buffaloes. These organisms are involved the formation of biofilm can evade treatment. There no definitive study to screen genes associated with production multocida. The present describes real-time PCR based approach for screening formation. Out 10 isolates screened formation, five them produced on Congo red agar which all resistant major antibiotics especially cotrimoxazole, nalidixic acid, enrofloxacin tetracyclines. All show presence indicating other factors influencing production. We suggest future studies may be targeted unravel influence