Evaluation of bacterial and plant-based expression systems for recombinant foot-and-mouth disease VP1 protein production
نویسندگان
چکیده
منابع مشابه
Plant-based expression systems for protein and antimicrobial peptide production
Molecular farming technology offers a unique advantage that almost any protein can be produced economically and safely under very controlled conditions. Besides traditional production systems, such as bacteria, yeasts, insects and mammal cell lines, plants can now be used to produce eukaryotic recombinant proteins, especially therapeutic ones. Their advantages as hosts for protein production in...
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Regarding high potential of green plants for development of recombinant vaccines, this research was conducted to evaluate expression of a novel recombinant vaccines against Foot and Mouth Disease (FMDV) in tobacco plant. For this purpose, a synthetic gene encoding 129-169 amino acids of foot and mouth disease virus capsid protein VP1 was transferred to tobacco plant via Agrobacterium-mediated g...
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Introduction Foot-and-mouth disease (FMD) is a highly contagious viral disease resulting in tremendous economical losses. In order to minimize the risks of an FMD outbreak in disease-free countries due to animal imports, rapid and reliable screening of sera from susceptible animals for the presence of FMD virus (FMDV) antibodies is necessary. The reasons for the development of a novel FMDV anti...
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Foot and Mouth Disease (FMD) is a very dangerous livestock disease which causes a serious loss in the production of milk and meat. Therefore, producing an effective recombinant subunit vaccine virus this disease is of great importance. Transient gene expression is a valuable tool to reach rapid and acceptable recombinant vaccine. An Agrobacterium-mediated transient gene expression assay was car...
متن کاملTransient Expression of Foot and Mouth Disease Virus (FMDV) Coat Protein in Tobacco (Nicotiana tabacom) via Agroinfiltration
Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants. Objective: The main goal of the present study was to eva...
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ژورنال
عنوان ژورنال: South African Journal of Botany
سال: 2013
ISSN: 0254-6299
DOI: 10.1016/j.sajb.2013.02.153