Evaluation of Anthelmintic Activity and GC-MS Characterization of Urochloa distachya (L.)

Background: The helminthiasis parasitic worm present in the gastrointestinal tract of animal cause infection and physiological damage. This occurs due to poor sanitation, impecunious personal hygiene, low socioeconomic position, lack education. Aim: Urochloa distachya (Poaceae) commonly called as signal grass used fodder for animal. investigation was undertaken investigate phytochemicals study anthelmintic activity. Materials Methods: plant material extracted by using petroleum ether methanol Soxhlet apparatus obtained extract subjected GC-MS analysis identification bioactive components responsible activity performed against earthworms (Eisenia fetida) at concentrations 12.5, 25, 50 mg/mL piperazine citrate standard. Results: showed five major ten minor compounds. compounds were identified Stigmasterol (14.76%); α-Amyrin (12.47%); γ-Sitosterol (10.31%); 17-(1,5-Dimethyl-3-phen ylthiohex-4-enyl) -4,4,10,13,14-pentamethyl 2,3,4,5,6,7,10,11,12-(9.12%) β-Amyrin (8.83%). D:C-Friedo-B': A'-neogammacer-9(11)-ene, 3-methoxy-, (3β)-(8.28%); Tritetracontane (7.23%); Campesterol (4.68%); Phytol (4.05%); Cholesterol (3.39%); Hexadecanoic acid, methyl ester (2.20%); 2-Pyrrolidinone, 1-methyl-(1.46%); Oleic 3-(octadecyloxy) propyl (1.27%); Squalene (0.84%) 9,19-Cyclolanostane-3,7-diol (0.68%). time taken paralysis death Eisenia fetida parasite noticed methanolic a concentration mg/kg compared standard drug. Conclusion: most achieved which could be attributed presence Octadecanoic acid. Also, active antimicrobial, antibacterial, antifungal, antiprotozoal activities ester; Phytol; Squalene; Cholesterol, γ-Sitosterol.