Evaluation of a new multi-epitope sequence of eight known Leishmania infantum antigens for HVL diagnosis by ELISA and Western blot
نویسندگان
چکیده
منابع مشابه
solution of security constrained unit commitment problem by a new multi-objective optimization method
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Design and optimization of Dot-blot ELISA system using specific antigens for simple and rapid diagnosis of glanders
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متن کاملEvaluation of an ELISA rapid device for the serological diagnosis of Leishmania infantum infection in dog as compared with immunofluorescence assay and Western blot.
In this study we compared a commercial enzyme linked immunosorbent assay (ELISA) rapid test (Snap CLATK Canine Leishmania Antibody Test Kit, IDEXX-Snap) with indirect immunofluorescence assay (IFA) and Western blot (WB) for the detection of Leishmania infantum antibodies in dogs. In total sera from 234 dogs were collected: 59 positives and 51 doubtful sera (IFA 1:40-1:80) from an L. infantum en...
متن کاملEvaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.
A Western blot method that uses antigens from culture promastigote forms of Leishmania (Viannia) braziliensis, L. (Leishmania) amazonensis, L. (Leishmania) tropica, and a trypanosomatid (strain 268T) isolated from naturally infected tomatoes was evaluated for laboratory diagnosis of American tegumentary leishmaniasis (ATL). Serum samples were obtained from 108 patients with ATL (group I), 23 ch...
متن کاملDiagnosis of infections with Leishmania infantum using PCR-ELISA.
On the basis of partial amplification of a cloned fragment of kDNA of Leishmania infantum which is specific for this species, we developed a PCR-ELISA technique which avoids the problems associated with classical diagnostic techniques. This technique was tested on 33 L. infantum strains from 19 different zymodemes, which were recognized equally. It was also used on human and canine clinical sam...
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ژورنال
عنوان ژورنال: Journal of Vector Borne Diseases
سال: 2021
ISSN: 0972-9062
DOI: 10.4103/0972-9062.318310