Evaluation of a new cytotoxicity assay forClostridium perfringenstype D epsilon toxin
نویسندگان
چکیده
منابع مشابه
cloning and expression of clostridium perfringenstype d vaccine strain epsilon toxin gene in e. coli as a recombinant vaccine candidate
background and objectives: clostridium perfringens, a gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. c. perfringens is subdivided into five types a to e based on its four major alpha, beta, epsilon and iota toxins. the aim of the present study was cloning and expression of c. perfringens type d vaccine strain epsilon...
متن کاملIn silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B
Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were...
متن کاملEpsilon-toxin plasmids of Clostridium perfringens type D are conjugative.
Isolates of Clostridium perfringens type D produce the potent epsilon-toxin (a CDC/U.S. Department of Agriculture overlap class B select agent) and are responsible for several economically significant enterotoxemias of domestic livestock. It is well established that the epsilon-toxin structural gene, etx, occurs on large plasmids. We show here that at least two of these plasmids are conjugative...
متن کاملSialidases Affect the Host Cell Adherence and Epsilon Toxin-Induced Cytotoxicity of Clostridium perfringens Type D Strain CN3718
Clostridium perfringens type B or D isolates, which cause enterotoxemias or enteritis in livestock, produce epsilon toxin (ETX). ETX is exceptionally potent, earning it a listing as a CDC class B select toxin. Most C. perfringens strains also express up to three different sialidases, although the possible contributions of those enzymes to type B or D pathogenesis remain unclear. Type D isolate ...
متن کاملRequirement of a conservative cytotoxicity assay selection in cellular experiments
Assessment of the cellular effects of natural and/or synthetic products is one of the major goals in biological experiments. This assessment may be conducted at different levels; cellular viability, cellular biological activity, and cellular structure and morphology. Different assays are available for any of above purposes. It is becoming of a routine exercise to apply any of these assays, and ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1994
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1994.tb06695.x