Evaluation of a multiplex PCR method to detect enteroaggregative Escherichia coli
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چکیده
منابع مشابه
Evaluation of a multiplex PCR method to detect enteroaggregative Escherichia coli.
Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator (aggR gene), the enteroaggregati...
متن کاملMultiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains.
We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC.
متن کاملIdentification of Biofilm Encoding Genes (agg) in the Escherichia coli Isolates by Multiplex-PCR Method
Introduction: Biofilm is a collection of microbial cells that are irreversibly suppressed and mildly washed away. The aggR gene is located on the main plasmid (pAA), which codes for many actuator factors. The pathogen strains of EAEC have an aggR gene, the gene has been identified in six classes of pathogenic E. coli. Materials & Methods: In the present study, 60 stool samples from children ...
متن کاملDevelopment of PCR for screening of enteroaggregative Escherichia coli.
In this study, we determined the sequence of the EcoRI-PstI fragment of the plasmid pCVD432, also termed the enteroaggregative Escherichia coli (EAggEC) probe. A primer pair complementary to this probe was designed for PCR amplification of a 630-bp region. Comparison of the analysis of the EAggEC probe sequence with those in database libraries revealed no significant similarity to any known bac...
متن کاملEnteroaggregative Escherichia coli.
Enteroaggregative Escherichia coli (EAEC) are an increasingly important cause of diarrhoea. E. coli belonging to this category cause watery diarrhoea, which is often persistent and can be inflammatory. EAEC have been implicated in sporadic diarrhoea in children and adults, in both developing and developed countries, and have been identified as the cause of several outbreaks worldwide. EAEC are ...
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ژورنال
عنوان ژورنال: BIOCELL
سال: 2006
ISSN: 1667-5746
DOI: 10.32604/biocell.2006.30.301