Evaluation of a Microplate Agglutination Test (MAT) for Serological Diagnosis of Canine Brucellosis
نویسندگان
چکیده
منابع مشابه
A Comparative Study of Microplate Agglutination Test (MAT) with Enzyme Linked Immunosorbant Assay (ELISA) for Diagnosis of Brucellosis
History of animal contact is expected in these profession i.e., agriculture, veterinary, dairy farming and animal husbandry. Brucellosis is a Zoonotic disease and is not uncommon in the rural population of our country. Brucellosis is one of the most common zoonosis and continues to be a major public health concern globally especially in the developing countries. The disease can be missed in the...
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Background: Brucellosis is a world-wide disease, which has a diverse clinical manifestation, and its diagnosis has to be proven by laboratory data. Serum agglutination test (SAT) is the most-widely used test for diagnosing brucellosis. The enzyme linked immunosorbent assay (ELISA) can also determine specific antibody classes against brucella. It is a sensitive, simple and rapid test, which coul...
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Background & Aims: Brucellosis is a public health problem in many developing countries, including Iran. Diagnosis is based on history, clinical manifestations and positive serum agglutination test (SAT) or Wright test. The purpose of this study was to evaluate the diagnostic value of SAT in pediatric Brucellosis. Methods: In this prospective study, during 2 years, 52 patients with clinical feat...
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Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods, including agglutination and gel diffusion tests. In this study, four recombinant antigens, heat shock protein 60, rhizopine-binding protein, Cu-Zn superoxide dismutase, and hypothetical protein (A...
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A modified rapid slide agglutination test for the presumptive identification of Brucella canis infection in dogs has been developed. The method required mixing 0.1 ml of canine serum with 0.1 ml of 0.2 M 2-mercaptoethanol solution. Equal volumes (0.05 ml) of the treated serum and the B. canis plate antigen were mixed. Agglutination results were read within 2 min. Clinical studies showed 100% ag...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2008
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.70.707