Evaluation of a genus reactive monoclonal antibody in rapid identification of Chlamydia trachomatis by direct immunofluorescence.

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Evaluation of a genus reactive monoclonal antibody in rapid identification of Chlamydia trachomatis by direct immunofluorescence.

This study compared the direct visualisation of elementary bodies in urogenital smears by the direct immunofluorescence test with the isolation of Chlamydia trachomatis in McCoy cell cultures treated with cytochalasin B. C trachomatis was isolated from 41 (16.4%) of 250 unselected specimens in McCoy cell cultures treated with cytochalasin B. Of the 41 culture positive specimens 37 (90%) were di...

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بررسی قدرت تشخیصی روشهای ایمونوفلئورسانس مستقیم( (DFAو رنگ آمیزی گیمسا در مقایسه با روش PCR ژن Omp1 جهت شناسایی کلامیدیا تراکوماتیس در نمونه ملتحمه بیماران مبتلا به کونژنکتیویت فولیکولار مراجعه کننده به بیمارستان فارابی در سال 1392

Background and Aim: Chlamydia trachomatis is an obligate intracellular parasite responsible for ocular and genital infections in human. Chlamydia trachomatis is the most common cause of chronic follicular conjunctivitis and is also responsible for 20% of acute conjunctivitis cases. As a rapid diagnosis is important in the reducing the long-term squeal of the diseases, the objective of this stud...

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Rapid identification of legionellae by a colony blot assay based on a genus-specific monoclonal antibody.

We recently developed a monoclonal antibody immunoglobulin G2a (2125) recognizing a genus-specific epitope on the 60-kDa heat shock protein of all Legionella species. In the current study, this antibody was used in a colony blot enzyme-linked immunosorbent assay for the rapid identification of Legionella cultures on agar plates. The whole protocol was completed in less than 2 h. All 59 Legionel...

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Rapid detection and identification of respiratory viruses by direct immunofluorescence.

The use of fluorescein-conjugated antiserum against respiratory syncytial (RS) and parainfluenza 1 and 3 viruses was compared with conventional techniques in the rapid detection of virus in tissue cultures inoculated with pharyngeal specimens known to contain these viruses. Twenty-three specimens were tested: 9 RS, 8 parainfluenza 1, and 6 parainfluenza 3. The fluorescent-antibody technique (FA...

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ژورنال

عنوان ژورنال: Sexually Transmitted Infections

سال: 1985

ISSN: 1368-4973

DOI: 10.1136/sti.61.4.252