Evaluation of a commercial ELISA for the specific detection of antibodies against Besnoitia besnoiti

Evaluation of a commercial ELISA for the specific detection of antibodies against Besnoitia besnoiti.

Bovine besnoitiosis is an economically important disease in cattle caused by the protozoan parasite Besnoitia besnoiti, which occurs endemically in many countries of Africa and Asia and is spreading in Europe. Serological identification of subclinically infected cattle is important to avoid the introduction of infected animals into naive herds. Here we determine the sensitivity and specificity ...

Prevalence of ELISA-detected specific antibodies against Besnoitia besnoiti in cattle of the Eastern and Southeastern Anatolian regions, Turkey

Background: Besnoitiosis caused by Besnoitia besnoiti is regarded as a re-emerging disease in cattle because of the increased number of cases and geographical distribution in many European countries. Aims: The present study was conducted to determine the presence of B. besnoiti in cattle in the Eastern and Southeastern Anatolia of Turkey. <str...

Evaluation of commercial ELISA kits for the detection of antibodies against bluetongue virus.

The aim of this study was to estimate the diagnostic value of different commercially available ELISA kits for the detection of bluetongue virus (BTV) antibodies in infected and vaccinated animals. The relative specificity of ELISA kits was evaluated using a panel of sera originating from healthy cattle, never vaccinated nor exposed to BTV. All ELISA kits applied had a high relative specificity ...

Comparative evaluation of immunofluorescent antibody and new immunoblot tests for the specific detection of antibodies against Besnoitia besnoiti tachyzoites and bradyzoites in bovine sera.

Besnoitia besnoiti, an apicomplexan parasite causes economically important disease in cattle in many countries of Africa and Asia is re-emerging in Europe. Serological identification of infected cattle is important because introduction of these animals into naive herds seems to play a major role in the transmission of the parasite. We report new, simplified immunoblot-based serological tests fo...

Standardization of ELISA for Detection of Avian Influenza Virus Antibodies