Estimation of Phytochemicals and Antioxidant potency of Trigonella foenum-graecum Leaves (Variety HM444)

Background: Plants contain phytochemicals that are crucial to their growth and development. The potential health benefits of dietary including flavonoids, isoflavones other polyphenols. Polyphenolic substances, the largest class phytochemicals, act as strong antioxidants due abundance hydroxyl groups. Trigonella foenum-graceum also known Fenugreek is rich source therefore possess extraordinary healing medicinal properties. Methods: present study designed evaluate chief phytochemical constituents antioxidant capacity acetone extract dried foenum-graecum leaves (Variety HM 444). like total phenolic content by Folin-Ciocalteu method, flavonoids AlCl3 colorimetric assay, sugars method Dubois reducing Nelson modified Somogyi determined in leaves. using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay phosphomolybdenum assay. Result: Various such (3.17 mg GAE/g), (1.34 CE/g), (32.35 mg/g), Reducing (0.53 mg/g) Non- (31.82 investigated activity evaluated reported an IC50 value 737.50 μg/mL. 28.21 AAE/g.