Estimation of cellulase production by Aureobasidium pullulans, Neurospora crassa, and Trichoderma reesei during solid and submerged state fermentation of raw and processed canola meal

In this study, cellulase, endoglucanase, and ?-glucosidase activity produced by three fungal strains (Aureobasidium pullulans, Neurospora crassa Trichoderma reesei) were determined on differently processed canola substrates [hexane extracted meal (HECM), washed HECM (WHECM), seed sprout (CSM)] in mono- co-culture fermentation under solid- submerged- state processes. Results showed that the inoculation yielded better results as compared to monocultures except for T. reesei which highest WHECM (440.02 U/g). Co-culture of A. pullulans + N. had cellulase CSM (1.89 FPU/g), (1.22 FPU/g); maximal endoglucanase at (HECM, 2.67 U/g), (CSM, 3.69 U/g) (WHECM, 3.21 These highlight impact various conditions microbial enzymatic activities, need strategically designed process optimal activities achieve fiber degradation meal.