Establishment of a Highly Efficient In Vitro Propagation System of Diospyros lotus

Persimmon (Diospyros) is an economically important tree widely cultivated for woody grain production in China, and Diospyros lotus mainly used as the grafting stock of persimmon. However, breeding stress-resistant rootstocks D. using molecular means has yet to be achieved; particular, efficient blade-regeneration system not been perfected date. This study examined effects different plant-growth regulators concentrations on primary culture stems with buds, induction leaf callus, differentiation adventitious shoots, rooting lotus. The optimal formula inducing axillary buds from was 1/2 Murashige Skoog (MS) medium containing 2.0 mg/L 6-benzylaminopurine (6-BA) 0.5 naphthaleneacetic acid (NAA), which rate approached 67.1%. best callus MS 6-BA NAA. Then transferred 6-(γ,γ-dimethylallylamino)purine (2iP), thidizuron (TDZ), 40 g/L sucrose induce shoots after dark 48 h, resulting 7.9 per explant a 75.2% frequency shoots. In addition, it difficult six times continuous transfer differentiation. were zeatin (ZT) 2iP proliferation culture, multiplication coefficient 7.6. inoculated into + 1.0 IBA NAA medium, 70.2%, average number heels 9.6. Thus, studies this area are expected facilitate rapid excellent growth, well theoretical support factory saplings’ care breeding.