Establishment of a dose-response curve for X-ray-induced micronuclei in human lymphocytes
نویسندگان
چکیده
منابع مشابه
Establishment of a Dose-response Curve for X-ray-Induced Micronuclei in Human Lymphocytes
The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is an established technique for biodosimetry. The aim of this project was to generate a X-ray induced micronuclei (MN) curve for peripheral blood lymphocytes taken from five healthy donors. The blood samples were irradiated with X-rays of 122 KeV at a dose rate of 0.652 Gy/min to doses of 0.5, 1, 2, 3, and 4 Gy. The blood ...
متن کاملDose estimation with the calibration of dose-response curve of micronucleus in human peripheral lymphocytes induced by 50MeV proton beams
Background: The purpose of this paper is to establish an easy and reliable biodosimeter protocol to evaluate the biological effects of proton beams. Materials and Methods: Human peripheral blood lymphocytes were irradiated using proton beams (LET: 34.6 keV μm−1), and the chromosome aberrations induced were analyzed using cytokinesis-blocked (CB) micronucleus (MN) assay. To determine th...
متن کاملdose estimation with the calibration of dose-response curve of micronucleus in human peripheral lymphocytes induced by 50mev proton beams
background: the purpose of this paper is to establish an easy and reliable biodosimeter protocol to evaluate the biological effects of proton beams. materials and methods: human peripheral blood lymphocytes were irradiated using proton beams (let: 34.6 kev μm−1), and the chromosome aberrations induced were analyzed using cytokinesis-blocked (cb) micronucleus (mn) assay. to determine the efficie...
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The effects of beta-carotene and ascorbic acid on spontaneous and x-ray-induced appearance of micronuclei (MNs) in human lymphocytes were studied. For 12 d, three groups of healthy volunteers were given beta-carotene-deficient meals containing 100 mg ascorbic acid. There was no supplementation in the first 6 d but, in the last 6 d, the respective groups were given beta-carotene (30 mg), ascorbi...
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ژورنال
عنوان ژورنال: Genome Integrity
سال: 2016
ISSN: 2041-9414
DOI: 10.4103/2041-9414.197162