Establishment of a clonal osteogenic cell line from newborn mouse calvaria
نویسندگان
چکیده
منابع مشابه
In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria
We investigated the capacity of a clonal osteogenic cell line MC3T3-E1, established from newborn mouse calvaria and selected on the basis of high alkaline phosphatase (ALP) activity in the confluent state, to differentiate into osteoblasts and mineralize in vitro. The cells in the growing state showed a fibroblastic morphology and grew to form multiple layers. On day 21, clusters of cells exhib...
متن کاملEstablishment of a Clonal Human Chondrosarcoma Cell Line
A clonal cell line with cartilage phenotypes and tumorigenicity during more than 3 years in culture was established from a human chondrosarcoma. In sparse cultures, the clona) line, named HCS-2/8, consisted of slightly elongated polygonal cells, which proliferated with a doubling time of 3.5 days. The cells became polygonal to spherical as they became confluent. After reaching confluence, the c...
متن کاملEstablishment a CHO Cell Line Expressing Human CD52 Molecule
Background: CD52 is a small glycoprotein with a GPI anchor at its C-terminus. CD52 is expressed by Normal and malignant T and B lymphocytes and monocytes. There are detectable amounts of soluble CD52 in plasma of patients with CLL and could be used as a tumor marker. Although the biological function of CD52 is unknown but it seems that CD52 may be involved in migration and activation of T-cells...
متن کاملBlood formation in a clonal cell line of mouse teratocarcinoma.
Pluripotent cells of a teratocarcinoma clonal line differentiate into various tissues when they are cultivated in submerged cultures. We describe here the formation of endodermal vesicles and blood islands in organotypic cultures of such cells. These are the first macroscopically visible tissues to appear in organ culture and they give rise to megalocytes, the ultimate stage of blood cell evolu...
متن کاملPrimary osteogenic sarcoma of the calvaria.
Osteogenic sarcoma of the skull is rare [1-7], particularly as a primary development in the later decades of life without associated Paget disease or history of irradiation. Because of the paucity of cases, the radiologic findings of calvarial osteogenic sarcoma have not been described in detail. Previous reports have dealt mainly with plain skull radiographs for the diagnosis of this tumor bef...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Oral Biology
سال: 1981
ISSN: 0385-0137
DOI: 10.2330/joralbiosci1965.23.899