Escherichia coli Soft Metal Ion-translocating ATPases

Families of soft-metal-ion-transporting ATPases.

Conservation of structure in proton-translocating ATPases of Escherichia coli and mitochondria.

arriving at (c ,+c ,+P) in the absence of antimycin must represent the oxidation of a second equivalent of QH, by the cyt. cI which remained oxidized after the first cycle of the complex. This second electron transfer presumably also occurs via the FeS centre, so that effectively the ‘invisible’ electron on the FeS centre gets replaced and pushed over on to cyt. c, in a second turnover of the c...

Genomic Comparison of Translocating and Non-Translocating Escherichia coli

Translocation of E. coli across the gut epithelium can result in fatal sepsis in post-surgical patients. In vitro and in vivo experiments have identified the existence of a novel pathotype of translocating E. coli (TEC) that employs an unknown mechanism for translocating across epithelial cells to the mesenteric lymph nodes and the blood stream in both humans and animal models. In this study th...

Metal ion dependence of recombinant Escherichia coli allantoinase.

Allantoinase is a suspected dinuclear metalloenzyme that catalyzes the hydrolytic cleavage of the five-member ring of allantoin (5-ureidohydantoin) to form allantoic acid. Recombinant Escherichia coli allantoinase purified from overproducing cultures amended with 2.5 mM zinc, 1 mM cobalt, or 1 mM nickel ions was found to possess approximately 1.4 Zn, 0.0 Co, 0.0 Ni, and 0.4 Fe; 0.1 Zn, 1.0 Co, ...

Dihydroorotase from Escherichia coli. Sulfhydryl group-metal ion interactions.

We have obtained 53 mg of 99% pure dihydroorotase from 10.9 g of frozen Escherichia coli pyrC plasmid-containing E. coli cells using a 4-step 16-fold purification procedure, a yield of 60%. We characterize the enzyme by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (a dimer of subunit molecular weight 38,300 +/- 2,900), high performance liquid chromatography gel sieving, amino acid ...