Escherichia coli shares T- and B-lymphocyte epitopes with Schistosoma mansoni
نویسندگان
چکیده
منابع مشابه
Schistosoma Mansoni Shares
The production of mAbs of various isotypes to Schistosoma mansoni has greatly contributed to the appreciation of the function of antibodies in the immunity against schistosomiasis, and has allowed the characterization of several potential protective antigens . Using this approach, we clearly established in previous studies (1, 2) the close relationship existing between a 38,000 Mr S. mansoni sc...
متن کاملIdentification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells.
Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind...
متن کاملHuman T- and B-cell responses to Schistosoma mansoni recombinant glyceraldehyde 3-phosphate dehydrogenase correlate with resistance to reinfection with S. mansoni or Schistosoma haematobium after chemotherapy.
Recently we reported that human T- and B-cell recognition of a 42-kDa protein (p42) in soluble extracts of adult Schistosoma mansoni worms correlates with resistance to reinfection with S. mansoni or S. haematobium. Amino acid microsequencing of p42 revealed that it consists predominantly of schistosome glyceraldehyde 3-phosphate dehydrogenase (SG3PDH). We have expressed SG3PDH in Escherichia c...
متن کاملSchistosoma mansoni shares a protective carbohydrate epitope with keyhole limpet hemocyanin
The glycanic epitope of the 38,000 Mr Schistosoma mansoni schistosomula major immunogen defined by the IPLSm1 protective mAb was identified in the hemocyanin of the marine mollusc Megathura crenulata, better known as KLH. This antigenic community was exploited to investigate further the biological properties of this epitope. KLH was shown to strongly inhibit the binding of IPLSm1 mAb to its 38,...
متن کاملExpression of recombinant antigens in Escherichia coli: application on immunochemical studies of Schistosoma mansoni tegumental antigens.
Sm15 and Sm13 are recognized by antibodies from mice protectively vaccinated with tegumental membranes, suggesting a potential role in protective immunity. In order to raise antibodies for immunochemical investigations, the genes for these antigens were expressed in pGEX and pMal vectors so that comparisons could be made among different expression systems and different genes. The fusion protein...
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ژورنال
عنوان ژورنال: The Journal of Infection in Developing Countries
سال: 2009
ISSN: 1972-2680,2036-6590
DOI: 10.3855/jidc.37