Erythropoietin exerts neuroprotective effect against hypoxic ischemic brain injury in neonatal mice by activating AMPK pathway and upregulating UCP2

Purpose: To investigate the neuroprotective effect of erythropoietin (EPO) against hypoxic-ischemic brain injury in neonatal mice, and underlying mechanism(s) action.
 Methods: Sixty mice were assigned to 3 groups: sham, model EPO intervention groups, each with 20 mice. Western blot assay was used determine changes protein expressions AMPK, UCP2 KLF2. Fluorescence intensity reactive oxygen species (ROS) evaluated flow cytometry.
 Results: The AMPK KLF2 cerebral tissues significantly up-regulated, relative (p < 0.05), while level also higher than 0.05). Mean fluorescence ROS cortex sham but it down-regulated group, group 0.05).
 Conclusion: exerts by activating pathway, up-regulating expression UCP2, inhibiting production mitochondrial ROS, reducing oxidative stress tissue. Thus, has potentials for use clinical practice as a agent.