Erythrocyte Protein Phosphorylation

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Phosphorylation in Erythrocyte M

Erythrocyte protein phosphorylation was from immunologically acquired spheroexamined in membrane preparations of cytosis cells or from cells from patients 25 patients with hereditary spherocytosis with hereditary elliptocytosis. Heating (HS). Reduced phosphorylation in submembranes to 45’C caused negligible strafe polypeptides was observed in 22 inhibition of membrane protein phosHS erythrocyte...

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Defining erythrocyte internal labeling by phosphorylation.

When erythrocytes are incubated with 32Pi, incorporation of label into phosphoproteins is a gradual process, increasing for at least 2 hours. Membrane phospholipids also are labeled. Exogenous protein kinase substrates are unlabeled in these incubations. This suggests that labeling by 32Pi occurs into polypeptides inside the erythrocytes. When erythrocytes are incubated with [gamma-32P]ATP and ...

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State of Spectrin Phosphorylation Does Not Affect Erythrocyte Shape or Spectrin Binding to Erythrocyte Membranes*

After equilibrating cells with [32P]phosphate there were 3.7 f 0.2 mol of [32P]phosphate/mol of Band 2 subunit; this is the total measured as nonradioactive phosphate in fresh cells (Harris, H. W., Wolfe, L. C., and Lux, S . E. (1978) Fed Roc. 37, 1507). This level can be reduced in intact cells by metabolic depletion, or on purified spectrin by tryptic removal of the terminal phosphorylated pe...

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State of spectrin phosphorylation does not affect erythrocyte shape or spectrin binding to erythrocyte membranes.

After equilibrating cells with [32P]phosphate there were 3.7 f 0.2 mol of [32P]phosphate/mol of Band 2 subunit; this is the total measured as nonradioactive phosphate in fresh cells (Harris, H. W., Wolfe, L. C., and Lux, S . E. (1978) Fed Roc. 37, 1507). This level can be reduced in intact cells by metabolic depletion, or on purified spectrin by tryptic removal of the terminal phosphorylated pe...

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State of Spectrin Phosphorylation Does Not Affect Erythrocyte Shape or Spectrin Binding to Erythrocyte Membranes*

After equilibrating cells with [32P]phosphate there were 3.7 f 0.2 mol of [32P]phosphate/mol of Band 2 subunit; this is the total measured as nonradioactive phosphate in fresh cells (Harris, H. W., Wolfe, L. C., and Lux, S . E. (1978) Fed Roc. 37, 1507). This level can be reduced in intact cells by metabolic depletion, or on purified spectrin by tryptic removal of the terminal phosphorylated pe...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1973

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)44313-5