Erratum to: “Morphological and Functional Heterogeneity of Zajdela Rat Ascites Hepatoma Cells”
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Insulin receptors in Zajdela rat ascites hepatoma cells and their sensitivity to certain enzymes and lectins.
The insulin receptors in two strains of Zajdela ascites hepatoma cells have been studied. Specific binding of insulin was investigated at 4,15, and 22°,and the amount of insulin bound at steady state was proportional to the cell concentration. The optimum pH was 7.6. Scatchard analysis of the insulin binding exhibited cur vilinear plots. The number of insulin receptors was mark edly reduced in...
متن کاملIntracellular synthesis of alpha-fetoprotein and fibrinogen without secretion by Zajdela rat ascites hepatoma cells.
Transplabtable Zajdela rat ascites hepatoma cells, previously considered "nonproducers," synthesize detectable amounts of intracellular alpha-fetoprotein (AFP) and fibrinogen, but fail to secret or release these serum proteins. Evidence for defective secretory mechanisms for serum proteins in these hepatoma cells (a) explains the failure to detect AFP in either the serum or ascitic fluid of rat...
متن کاملIntracellular Synthesis of a-Fetoprotein and Fibrinogen without Secretion by Zajdela Rat Ascites Hepatoma Cells1
Hepatoma Cell Protein Synthesis in Vitro. Zajdela ascites hepatoma cells (C strain) (28) were transplanted i.p. into adult male Charles RivenCD rats, and cells were harvested 6 to 7 days later. Hepatoma cells were separated from the ascitic fluid by centnifugation at 50 x g for 15 mm at 5°, then were washed 3 times with 0.9% NaCI solution . For the short term synthesis studies, hepatoma cells ...
متن کامل[Growth of Zajdela ascites hepatoma cells in vivo following treatment with trypsin and pronase in vitro].
Im Rahmen einer breit angelegten Untersuchungsreihe über Antigenstrukturen an Tumorzelloberflächen behandelten wir unter anderem Zajdela-Asciteshepatomzellen in vitro mit geringen Konzentrationen an Trypsin und Pronase und prüften das Angehen der Tumoren nach Wiederverimpfung dieser Zellen an Ratten im Vergleich zu nicht enzymierten Kontrollzellen. Veranlassung für die Publikation dieser Teiler...
متن کاملproten tyrosine phosphatase activity in rat ascites hepatoma cells
protein tyrosine phosphatases (ptpases) regulate tyrosine phosphorylation of target proteins involved in several aspects of cellular functions. enzyme activities of the ptpases in cytosolic and particulate fractions of rat ascites hepatoma cell lines were determined and compared with those of normal rat liver. our present data revealed that although there was no neoplatic-specific alteration of...
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ژورنال
عنوان ژورنال: Cell and Tissue Biology
سال: 2010
ISSN: 1990-519X,1990-5203
DOI: 10.1134/s1990519x10060131