Ergosterol and Lanosterol from Aspergillus nidulans
نویسندگان
چکیده
منابع مشابه
Aspergillus nidulans
Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veAl) , which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veAl that restore the light-dependent conidiation phenotype. Alle...
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Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifugation was applied. The resulting nuclei were of good purity and morphological s...
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Sterols are widely distributed in nature. It has been suggested that they be classified into three groups according to their occurrence : (1) zoosterols, the animal sterols (cholesterol, coprosterol) ; (2) phytosterols, the plant or vegetable sterols (sitosterol, stigmasterol) ; and (3) mycosterols, the sterols found in fungi (ergosterol, fungisterol). Although ergosterol has been detected in s...
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Mitosis in growing hyphae of haploid and diploid Aspergillus nidulans has been studied with the light microscope in Helly-fixed preparations. At the periphery of the nucleus a sharply defined granule is invariably found which has strong affinity for acid fuchsin. Mitosis begins with the duplication of this granule and the development inside the nucleus of a gradually lengthening fibre between t...
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Aspergillus nidulans strain G191 was transformed to hygromycin resistance using plasmid pDH25, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the A. nidulans trpC gene. Southern hybridizations of transformants revealed multiple, integrated copies of the vector. A pleiotropic effect conferring increased hygromycin B sensitivity was found to ...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1982
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-128-5-1053