Epidemiological evaluation of a monoclonal ELISA detecting antibodies against bovine leukaemia virus in serum pools
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منابع مشابه
Using various antigen preparations to produce monoclonal antibodies against bovine leukaemia virus (BLV) gp51SU
The objective of this study was to compare different antigen preparations to produce monoclonalantibodies against bovine leukaemia virus gp51SU. The four antigen preparations for immunization ofBALB/c mice were: CL: BLV-FLK cell lysate, UF: a fraction of CL (between 30 and 100 kDa), WVP: wholevirus particles and SP: with ion exchange chromatography, gp51SU was semipurified. A total of ninesucce...
متن کاملusing various antigen preparations to produce monoclonal antibodies against bovine leukaemia virus (blv) gp51su
the objective of this study was to compare different antigen preparations to produce monoclonalantibodies against bovine leukaemia virus gp51su. the four antigen preparations for immunization ofbalb/c mice were: cl: blv-flk cell lysate, uf: a fraction of cl (between 30 and 100 kda), wvp: wholevirus particles and sp: with ion exchange chromatography, gp51su was semipurified. a total of ninesucce...
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A number of mouse x mouse hybridomas producing monoclonal antibodies (MAbs) against bovine growth hormone (bGH) were prepared by fusion of spleen cells from bGH-primed mice (Balb/c) with non-secretory mouse myeloma cells (PAIOP3) and characterized. MAbs obtained from three fusion experiments belonged to IgM, IgG1 and IgG2b class/subclass of antibodies. Cross-reaction studies indicated that gene...
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Background and Aims: Vaccine is available, but fetal infection with rubella virus is still a main cause of congenital birth defects and mental retardation in many countries. Mass vaccination campaigns and Expanded Program of Immunization (EPI) have increased vaccine coverage in the world with a substantial impact on the reduction of rubella infections, such as Congenital Rubella Syndrome (CRS)....
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Since April 2010, Tembusu virus (TMUV) which is a contagious pathogen of waterfowls, causing symptoms of high fever, loss of appetite and fall in egg production, has been reported in east of China. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) which detects for TMUV was developed, using two monoclonal antibodies (mAbs) against the TMUV envelope (E) protein. BALB/c mic...
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ژورنال
عنوان ژورنال: Epidemiology and Infection
سال: 1994
ISSN: 0950-2688,1469-4409
DOI: 10.1017/s0950268800068588