Enzymic activities of two-chain pepsinogen, two-chain pepsin, and the amino-terminal lobe of pepsinogen.
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چکیده
منابع مشابه
Enzymic Dephosphorylation of Pepsin and Pepsinogen
It has been shown by the work presented in this paper that it is possible to dephosphorylate enzymically pepsin and pepsinogen with a variety of phosphatases. With the aid of a phosphodiesterase and the prostate phosphatase it has been established that the phosphorus in the two proteins is present as a diester and connects two sites of the peptide chain in a cyclic configuration. Removal of the...
متن کاملPepsinogen and Pepsin
Evidence relating to the structure and properties of swine pepsinogen and pepsin has been reviewed and used to suggest a tentative two dimensional picture of the skeleton of these two proteins. When pepsinogen, a folded single peptide chain, is converted to pepsin, there is a profound change in the physical and chemical properties of the protein. In an as yet unknown manner, except that it is i...
متن کاملBovine Pepsinogen and Pepsin
As the first step in the investigation of the structure and action of little known gastric zymogens and enzymes, pepsinogen has been isolated from the mucosa of the fourth stomach (abomasum) of the’ cow. The pepsinogen was purified by ammonium sulfate fractionation, batch absorption on diethylaminoethyl (DEAE) cellulose, recycling gel filtration on Sephadex G-100, and finally chromatography on ...
متن کاملThe amino-terminal sequence of porcine pepsinogen.
Amino acid sequence studies were performed on (a) peptides obtained from tryptic hydrolysates of succinyl pepsinogen and (b) those isolated from a peptide mixture formed when pepsinogen is activated to pepsin. These peptides taken together with tryptic peptides obtained from reduced carboxymethylated pepsinogen establish the complete sequence of 41 residues at the NH&erminal end of the single p...
متن کاملPepsinogen and Pepsin
Evidence relating to the structure and properties of swine pepsinogen and pepsin has been reviewed and used to suggest a tentative two dimensional picture of the skeleton of these two proteins. When pepsinogen, a folded single peptide chain, is converted to pepsin, there is a profound change in the physical and chemical properties of the protein. In an as yet unknown manner, except that it is i...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)41920-5