ENZYME KINETICS IN A HISTOCHEMICAL SYSTEM
نویسندگان
چکیده
منابع مشابه
The Kinetics of Enzyme Mixtures
Even purified enzyme preparations are often heterogeneous. For example, preparations of aspartate aminotransferase or cytochrome oxidase can consist of several different forms of the enzyme. For this reason we consider how different the kinetics of the reactions catalysed by a mixture of forms of an enzyme must be to provide some indication of the characteristics of the species present. Based...
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This paper describes a microanalytical method for determining enzyme kinetics using a continuous-flow microfluidic system. The analysis is carried out by immobilizing the enzyme on microbeads, packing the microbeads into a chip-based microreactor (volume approximately 1.0 nL), and flowing the substrate over the packed bed. Data were analyzed using the Lilly-Hornby equation and compared to value...
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Histochemical observations were carried out on various hydrolytic enzymes in the spontaneous mammary carcinoma of C3H-strain mice. Alkaline phosphatase activity was observed in the luminal border of the tubular structure and in the periluminal surface, which showed a cystic dilatation in ducts and papillae. Capillaries in the stroma showed a high enzymatic activity. Acid phosphatase activity wa...
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A certain Markov chain which was encountered by T. L. Hill in the study of the kinetics of a linear array of enzymes is studied. An explicit formula for the steady state probabilities is given and some conjectures raised by T. L. Hill are proved.
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1958
ISSN: 0022-1554,1551-5044
DOI: 10.1177/6.6.431