Enzyme immunoassay for the detection of Chlamydia trachomatis antigen in urethral and endocervical swabs.
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چکیده
منابع مشابه
Enzyme immunoassay for the detection of Chlamydia trachomatis antigen in urethral and endocervical swabs.
An enzyme immunoassay technique based on the direct detection of Chlamydia trachomatis antigen in urethral or cervical swabs was used for the rapid diagnosis of chlamydial genital infection. Urethral and cervical samples from 140 patients were tested in parallel by enzyme immunoassay and cell culture using iodine staining. The direct test had a sensitivity of 92.5% and specificity of 97.2% when...
متن کاملComparison of a polymer conjugate-enhanced enzyme immunoassay to ligase chain reaction for diagnosis of Chlamydia trachomatis in endocervical swabs.
Two endocervical swabs from each of 1,123 women were collected into manufacturer-supplied transport tubes and tested for Chlamydia trachomatis by a polymer conjugate-enhanced (PCE) enzyme immunoassay (EIA) (IDEIA PCE Chlamydia; DAKO) and a ligase chain reaction assay (LCx Chlamydia; Abbott). After confirmation by the EIA blocking test, the sensitivity of the IDEIA PCE remained at 91.8% and the ...
متن کاملMulticenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs.
The performance of the Becton Dickinson BDProbe Tec ET System Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assays (BD Biosciences, Sparks, Md.) was evaluated in a multicenter study. Specimens were collected from 2,109 men and women, with or without symptoms, attending sexually transmitted disease, family planning, and obstetrics and gynecology clinics. Both swab and urine sampl...
متن کاملDetection of Chlamydia trachomatis endocervical infections by ligase chain reaction versus ACCESS Chlamydia antigen assay.
Ligase chain reaction (LCR) was compared with ACCESS immunoassay for detection of chlamydial infections in females. Despite efforts to improve ACCESS performance by evaluation of specimens that were in the test performance "grey zone," LCR remained more sensitive and was less expensive to perform. ACCESS had a sensitivity of 83.9%, a specificity of 99.7%, a positive predictive value of 96.3%, a...
متن کاملGenotyping of Chlamydia trachomatis from endocervical specimens in Shiraz, Iran
The objective of this study was to determine the prevalence and distribution of Chlamydia trachomatisgenotypes in Shiraz, Iran. Two hundred twelve cervical swab samples were collected from women attendingShahid Motahari Polyclinic in Shiraz, Iran. The endocervical specimens were screened for C. trachomatis byplasmid PCR. Genotyping was performed in C. trachomatis-positive samples by nested PCR ...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1985
ISSN: 0021-9746
DOI: 10.1136/jcp.38.7.740